treatment with endoh (New England Biolabs)
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Structured Review
New England Biolabs
treatment with endoh
Treatment With Endoh, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/endo+h+treatment/Endo+H/bio_rxiv__64898__2026__02__27__708584-187-19-22
Average 99 stars, based on 4403 article reviews
Treatment With Endoh, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4403 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/endo+h+treatment/Endo+H/bio_rxiv__64898__2026__02__27__708584-187-19-22
Average 99 stars, based on 4403 article reviews
treatment with endoh - by Bioz Stars,
2026-09
99/100 stars
Images
1) Product Images from "The zinc metalloprotease ZMPSTE24 binds a distinct topological isoform of the tail-anchored protein IFITM3"
Article Title: The zinc metalloprotease ZMPSTE24 binds a distinct topological isoform of the tail-anchored protein IFITM3
Journal: bioRxiv
doi: 10.64898/2026.02.27.708584
Figure Legend Snippet: HEK293 cells were transfected with Flag-tagged ZMPSTE24 E336A and myc-IFITM3-opsinC (left panels) or Flag-tagged IFITM3 and myc-IFITM3-opsinC (right panels). Proteins were immunoprecipitated with anti-Flag agarose and then mock treated or treated with EndoH or PNGase prior to SDS-PAGE and western blotting. Positions of the glycosylated isoforms of IFITM3-opsinC are indicated. IFITM3-opsinC co-precipitated by ZMPSTE24 E336A was EndoH-and PNGase-sensitive. A glycosylated species of IFITM3-opsinC co-precipitated by Flag-IFITM3 was EndoH-resistant (compare lanes 4 and 5), indicating it had been modified by Golgi-localized glycosyltransferases.
Techniques Used: Transfection, Immunoprecipitation, SDS Page, Western Blot, Modification
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